A lateral flow protein microarray for rapid and sensitive antibody assays

Abstract

Protein micro arrays are useful tools for highly multiplexed determination of presence or levels of clinically relevant biomarkers in human tissues and bio fluids. However, such tools have thus far been restricted to laboratory environments. Here, we present a novel 384-plexed easy to use lateral flow protein micro array device capable of sensitive (<30 ng/mL) determination of antigen-specific antibodies in ten minutes of total assay time. Results were developed with gold nano beads and could be recorded by a cell-phone camera or table top scanner. Excellent accuracy with an area under curve (AUC of 98% was achieved in comparison with an established glass micro array assay for 26 antigen-specific antibodies. We propose that the presented framework could find use in convenient and cost-efficient quality control of antibody production, as well as in providing a platform for multiplexed affinity-based assays in low-resource or mobile settings. © 2011 by the authors; licensee MDPI, Basel, Switzerland.