Medium strength in inorganics and PVP concentration effects on cherry rootstocks in vitro rooting

Abstract

The effects of two strength media (full and half) in inorganics and five polyvinylpyrrolidone (PVP) concentrations combined with 1 mg/l indole-3-butyric acid on the in vitro rooting of the CAB-6P, Gisela 6 and M × M 14 cherry rootstocks were investigated. For the CAB-6P, root number (5.80) was greatest by adding 5 g/l PVP to the half strength medium and 1 g/l PVP resulted in 80% rooting percentage. For Gisela 6, 1 g/l PVP in half strength medium led to the highest root number (6.30) as well as the highest rooting percentage (90.91%). On the contrary, root length was maximum (36.17 mm) in the full strength medium without adding PVP. In M × M 14, root number was maximum (8.33) in the PVP-free full strength medium, rooting percentage was 100% by adding 5 g/l PVP to either full or half strength medium and root length was greatest by incorporating 1 or 2.5 g/l PVP into the half strength medium. PVP seems to be a promising rooting agent in tissue culture systems.