Stimulus-specific hierarchy of enhancer elements within the rat prodynorphin promoter

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Abstract

Induction of the prodynorphin gene occurs in a tissue-specific manner following different physiological stimuli. Using electrophoretic mobility shift assays, we studied the relative activity of the five major regulatory sites in the rat prodynorphin promoter. Prodynorphin cyclic AMP-responsive element 2 (DynCRE2), DynCRE3, and the noncanonical prodynorphin AP-1 (ncDynAP-1) regulatory sites control, in a coordinated manner, prodynorphin induction in the spinal cord after noxious stimulation, whereas prodynorphin up-regulation in supraoptic neurons is regulated predominantly by the ncDynAP-1. Conversely, prodynorphin transactivation in the ovaries upon gonadotropin stimulation is controlled by DynCRE1 and DynCRE3. Our results support the idea that stimulus-specific changes in nuclear proteins establish a functional hierarchy among regulatory sites in the prodynorphin promotor and provide further insight in the molecular mechanisms that govern prodynorphin gene regulation.

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APA

Carrión, A. M., Mellström, B., Luckman, S. M., & Naranjo, J. R. (1998). Stimulus-specific hierarchy of enhancer elements within the rat prodynorphin promoter. Journal of Neurochemistry, 70(3), 914–921. https://doi.org/10.1046/j.1471-4159.1998.70030914.x

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