Tissue microarrays (TMAs) enable high-throughput tissue analysis by selecting a large number of paraffin-embedded donor tissue block cores and transferring these tissue cores into a positionally encoded array in the recipient TMA block. Once TMAs are constructed, a variety of analysis may be performed on the arrays including histochemical, immunohistochemical, or immuno fluorescent staining, and in situ hybridization for DNA or RNA. TMAs offer a cost-effective method for performing parallel analysis of a large number of tissue samples. In this chapter we outline the method of TMA construction with an emphasis on providing useful information in the analysis of a variety of pancreatic neoplasms, including pancreatic adenocarcinomas and pre-invasive lesions. The technique of TMA construction in this chapter is restricted to the use of formalin-fixed paraffin-embedded tissue. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Remotti, H. (2013). Tissue microarrays: Construction and use. Methods in Molecular Biology, 980, 13–28. https://doi.org/10.1007/978-1-62703-287-2_2
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