Metabolism of pre-messenger RNA splicing cofactors: Modification of U6 RNA is dependent on its interaction with U4 RNA

Abstract

The requirements for the formation of pseudouridine (Ψ) in U4 and U6 RNAs, cofactors in the splicing of pre-messenger RNA, were investigated in vitro using HeLa nuclear (NE) and cytoplasmic (S100) extracts. Maximal Ψ formation for both RNAs was extract order-dependent. Maximal Ψ formation in U4 RNA required incubation in S100 followed by the addition of NE, paralleling the in vivo maturation pathway of U4 RNA. In contrast, maximal formation of Ψ in U6 RNA required incubation in NE followed by the addition of S100 extract. Since U6 RNA does not exit the nucleus in vivo the contribution of S100 was investigated. In experiments where the extracts were treated with micrococcal nuclease to digest endogenous snRNAs, the efficient formation of Ψ in U6 RNA was dependent on the presence of U4 RNA, but not in U5 RNA or tRNA. When mutant U4 RNAs that inhibit or strengthen the interaction between U4 RNA and U6 RNA were substituted for wild-type U4 RNA, the results confirmed the need for the interaction between these two RNAs for Ψ formation in U6 RNA. U6 RNA isolated from glycerol gradients after incubation in extracts had four times as much Ψ when associated with U4 RNA.