3D brain organoids derived from human pluripotent stem cells (hPSCs) possess the remarkable ability to self-organize and differentiate into tissue resembling the early human fetal brain. Brain organoids provide a powerful tool for studying human brain development and disease in an in vitro system. Here we describe a protocol for the differentiation of hPSCs to human cerebral organoids using a commercially available kit (STEMdiff™ Cerebral Organoid Kit) and discuss methods to scale up the protocol in a high-throughput manner.
CITATION STYLE
Chew, L., Añonuevo, A., & Knock, E. (2022). Generating Cerebral Organoids from Human Pluripotent Stem Cells. In Methods in Molecular Biology (Vol. 2389, pp. 177–199). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1783-0_15
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