We studied the expression of normal cellular prion protein (PrPC) in mouse lymphoid tissues with newly developed mAbs to PrPC. Most of the mature T and B cells in the peripheral lymphoid organs do not express PrPC. In contrast, most thymocytes are PrPC+. In the bone marrow, erythroid cells and maturing granulocytes are PrPC+. Approximately 50% of the cells in the region of small lymphocytes and progenitor cells also express PrPC. Most of these PrPC+ cells are CD43+, but B220−, surface IgM− (sIgM−), and IL-7R−, a phenotype that belongs to cells not yet committed to the B cell lineage. Another small group of the PrPC+ cell are B220+, and some of these are also sIgM+. The majority of the B220+ cells, however, are PrPC−. Therefore, PrPC is preferentially expressed in early bone marrow progenitor cells and subsets of maturing B cells. Supporting this interpretation is our observation that stimulation of bone marrow cells in vitro with PMA results in a decrease in the number of PrPC+B220− cells with a corresponding increase of sIgM+B220high mature B cells. This result suggests that the PrPC+B220− cells are potential progenitors. Furthermore, in the bone marrow of Rag-1−/− mice, there are an increased number of PrPC+B220− cells, and most of the developmentally arrested pro-B cells in these mice are PrPC+. Collectively, these results suggest that PrPC is expressed preferentially in immature T cells in the thymus and early progenitor cells in the bone marrow, and the expression of PrPC is regulated during hemopoietic differentiation.
CITATION STYLE
Liu, T., Li, R., Wong, B.-S., Liu, D., Pan, T., Petersen, R. B., … Sy, M.-S. (2001). Normal Cellular Prior Protein Is Preferentially Expressed on Subpopulations of Murine Hemopoietic Cells. The Journal of Immunology, 166(6), 3733–3742. https://doi.org/10.4049/jimmunol.166.6.3733
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