Molecular characterization of fluoroquinolone resistance mechanisms of Campylobacter isolates from duck meats

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Abstract

The purpose of this study was to identify the molecular basis of quinolone resistance of Campylobacter isolates recovered from duck meats. Sixty-one isolates from duck meat samples were studied using sequence analysis of the gyrA gene, and PCR assays were used to identify the presence of the CmeABC efflux pump and its restored sensitivity in the presence of efflux-pump inhibitors. High-level resistance to nalidixic acid and ciprofloxacin was attributed to amino acid substitutions Thr-86-Ile in some isolates. The PCR assay confirmed the presence of the cmeB gene in 29 (47.5%) of the 61 Campylobacter isolates. Phenylalanine arginine β-naphthylamide reduced the MICs of ciprofloxacin and nalidixic acid in 16 (55.2%) and 26 (89.7%) isolates, respectively. The Thr-86-Ile substitution in the gyrA was the primary contributor to the high-level quinolone resistance in Campylobacter isolates from duck meats.

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APA

Kang, M., Wei, B., Choi, S. W., Cha, S. Y., & Jang, H. K. (2017, December 1). Molecular characterization of fluoroquinolone resistance mechanisms of Campylobacter isolates from duck meats. Journal of Food Protection. International Association for Food Protection. https://doi.org/10.4315/0362-028X.JFP-17-219

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