Inflammasomes are large multiprotein complexes that assemble in response to cellular stress and infection. NOD-like receptor-related proteins (NLRPs) are essential components of these complexes and are activated by exogenous and endogenous danger signals such as crystalline substances, extracellular ATP, and pore-forming toxins. In general, inflammasome activation is accompanied by perturbations in cellular homeostasis. For example, most inflammasome activators will trigger cation efflux, reactive oxygen species (ROS) generation and caspase-1-dependent cell death, commonly referred to as pyroptosis. In this chapter, we describe protocols to examine infl ammasome activation and accompanying events in vitro.
CITATION STYLE
Cunningham, C. C., Corr, E. M., Cox, D. J., & Dunne, A. (2015). Investigating inflammasome activation under conditions of cellular stress and injury. Methods in Molecular Biology, 1292, 105–112. https://doi.org/10.1007/978-1-4939-2522-3_8
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