Nontemplated nucleotide addition prior to polyadenylation: A comparison of Arabidopsis cDNA and genomic sequences

Abstract

A comparison of Arabidopsis DNA sequences revealed that the final nucleotides at the 3′ end of approximately half of the Arabidopsis mRNAs, immediately upstream of the poly(A) tail, differ from the corresponding genomic sequences. This suggests that extra nucleotides were added to these mRNAs at their 3′ termini prior to polyadenylation. Among the mRNAs containing additional nucleotides, approximately 65% had a single additional nucleotide, with the nucleotide C added most often. This nontemplated addition before the addition of the poly(A) sequence could be a major contributing factor to the often observed heterogeneity in transcription products. These findings should be helpful in the elucidation of the mechanisms of mRNA 3′-end processing.