The lipogenic enzymes fatty acid synthase (FAS; EC 2.3.1.85), citrate cleavage enzyme (CCE; EC 4.1.3.8), malic enzyme (ME; EC 1.1.1.40). glucose-6-phosphate dehydrogenase (G6PDH; EC 1.1.1.49) and 6-phosphogluconate dehydrogenase (PGDH; EC 1.1.1.44) were investigated in liver and in brown adipose tissue (BAT) of Wistar rats under various dietary conditions and in the presence of 15 to 250 ppm (approximately 0.045-0.75 µmol/kg chow) polychlorinated biphenyls (PCBs). In response to refeeding starved animals, enzyme activities in both tissues increased to above normal levels and thereafter exhibited pronounced oscillations of their, activities. The extent of increase depended on the carbohydrate and fat content of the, diet. The lipogenic enzymes could be grouped in two categories according to their sensitivity to dietary carbohydrate: FAS and CCE responded faster to smaller changes in dietary composition, while ME, G6PDH and PGDH required larger changes and more time to, respond. Diet-induced alterations of enzyme activities were of the same order of magnitude in liver and BAT They were age-dependent, being more pronounced in young, animals. Independent of the type of dietary manipulations, activities changed in a coordinate fashion, i.e., the changes of the activities of all 5 enzymes occurred at similar ratios to each other with an identical time, course. Feeding PCB-containing diets resulted in a considerable increase of the activities of the lipogenic enzymes in liver, which was significantly greater with ME, G6PDH and, PGDH. The effect was dose-dependent but, transient. In liver the response to PCB feeding was identical in male and female animals, whereas in BAT lipogenic activities increased in females, but decreased in, males. Refeeding starved animals with a PCB-containing diet led to an additional stimulation of the normal refeeding-induced increase of the enzyme activities in liver and, BAT. This PCB-induced increase was 2-fold for FAS and CCE, but up to 15-fold for the other, enzymes. All PCB-induced effects were significantly less pronounced in old than in young, animals. In primary hepatocytes activities increased in hormone-free medium in the presence of, PCBs. While activity was induced in insuline- and triiodothyronine-containing medium, this increase was significantly greater with PCBs, present. © 1994 Verlag der Zeitschrift für Naturforschung. All rights reserved.
CITATION STYLE
Boll, M., Lutz, L. W., Weber, D., Stampfl, A., & Messner, B. (1994). Lipogenic Enzymes of Rat Liver and Adipose, Tissue. Dietary Variations and Effect of Polychlorinated Biphenyls. Zeitschrift Fur Naturforschung - Section C Journal of Biosciences, 49(9–10), 665–678. https://doi.org/10.1515/znc-1994-9-1018
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