Determination of 14 chemical constituents in the traditional Chinese medicinal preparation Huangqin-Tang by high performance liquid chromatography

Abstract

The high performance liquid chromatographic (HPLC) method for the identification and determination of baicalin (BG), wogonoside (WG), oroxylin-A-glucoside (OG), baicalein (B), wogonin (W), orxylin-A (O), paeoniflorin (PF), glycyrrhizic acid (GL), glycyrrhetinic acid (GA), liquiritin (LG), isoliquirition (ILG), liquiritigenin (L), isoliquiritigenin (IL) and ononin (ON) in Huangqin-Tang was established. The samples were separated with a Wakosil C18 column (4.6 × 150 mm) by linear gradient elution using A (MeOH-HAC 100: 1, v/v)-B (Water-HAC 100: 1, v/v) (0 min, 30 : 70; 15 min, 40 : 60; 30 min, 60 : 40; 45 min, 80 : 20; 60 min, 100 : 0) as the mobile phase at a flow-rate of 1.0 ml/min. The detection was by diode-array UV/Vis detector (DAD), and the wavelength was set at the range of 200-400 nm. Satisfactory results were obtained within 60 min for the simultaneous determination of the 14 constituents. The repeatability (RSD) of the method was generally less than 2% (n=5, interday and intraday). The recovery of BG was 96.9±1.71, WG was 98.9±2.99, PF was 99.7±0.52, LG was 95.3±2.67, GL was 96.7±3.44, and GA was 94.8±4.16, respectively.