Trans-splicing is a mechanism by which two pre-mRNAs are processed to produce a mature transcript that contains exons from both precursors. This process has been described mostly in trypanosoma, nematodes, plant/algal chloroplasts and plant mitochondria [Bonen et al (1993) FASEB J. 7. 40-46]. Our studies clearly demonstrate that a trans-splicing reaction occurs in the processing of the carnitine octanoyltransferase (COT) gene in rat liver. Three different mature transcripts of COT have been found in vivo, the canonical cis-spliced mRNA and two trans-spliced transcripts, in which either exon 2 or exons 2 and 3 are repeated. Splicing experiments in vitro also indicate the capacity of exon 2 to act either as a donor or as an acceptor of splicing, allowing the trans-splicing reactions to occur. © Springer Science+Business Media New York 1999.
CITATION STYLE
Caudevilla, C., Serra, D., Miliar, A., Codony, C., Asins, G., Bach, M., & Hegardt, F. G. (2000). Processing of carnitine octanoyltransferase pre-mRNAs by CIS and trans-splicing. Advances in Experimental Medicine and Biology, 466, 95–102. https://doi.org/10.1007/0-306-46818-2_10
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