Simultaneous determination of oxyresveratrol and resveratrol in rat bile and urine by HPLC after oral administration of smilax china extract

Abstract

Oxyresveratrol (trans-2,4,3′,5′-tetrahydroxystilbene, OXY) and resveratrol (trans-3,5,4′-trihydroxystilbene, RES) are the two most important constituents of the traditional Chinese medicine Smilax china. A reversed-phase high-performance liquid chromatographic method was developed to determine OXY and RES in rat bile and urine after oral administration of Smilax china extract. The biological samples were analyzed by HPLC on Aglient Zorbax SB-C18 column (250 x 4.6 mm, 5 μm) at a wavelength 320 nm and at a flow rate of 1.0 mL/min. The method was accurate and reproducible for determination. The cumulative excretion of OXY and RES was 0.29% and 0.97% in bile samples, 0.84% and 0.65% in urine samples, respectively.