Isolates characterization of Xanthomonas sp. Associated with rice seed and differentiation of Xanthomonas oryzae strains

Abstract

Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc), causal agents of bacterial leaf blight (BLB) and bacterial leaf streak (BLS), respectively, are pests transmitted by seeds and suffer restriction on international rice trade, because are absent in many countries and have potential to compromise seriously rice production. Twenty-one isolates from rice seeds from Uruguay and Argentina, Gram negative, oxidase positive, with typical yellow pigmentation of genus Xanthomonas and positive in ELISA for Xoo, were characterized to determine their association with X. oryze pathovars. Isolates were characterized by biochemical, biological and antibiogram methods. PCR and qPCR analyzes were performed to confirm genus Xanthomonas and identification of Xoo and Xoc. Characterization of 21 Xanthomonas sp. isolates positive on ELISA to Xoo, showed variability among isolates and distinction of Xo.