NMR-based metabolomics is an established technique for characterizing the metabolite profile of biological fluids and investigating how metabolite profiles change in response to biological and/or clinical stimuli. Thus, NMR-based metabolomics has the potential to discover biomarkers for diagnosis, prognosis, and/or therapy of clinical conditions, as well as to unravel the physiology underlying clinical conditions. Here, we describe a detailed protocol for NMR-based metabolomics of oral biofluids, including sample collection, sample handling, NMR data acquisition, and processing. In addition, we give a general overview of the statistical analysis of the resulting metabolomic data.
CITATION STYLE
Schirra, H. J., & Ford, P. J. (2017). NMR-based metabolomics of oral biofluids. In Methods in Molecular Biology (Vol. 1537, pp. 79–105). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6685-1_5
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