Polymerase chain reaction (PCR) amplification of antibiotic resistance genes as well as staphylococcal cassette chromosome mec (SCCmec) typing and pulsed-field gel electrophoresis (PFGE) of SmaI macrorestriction fragments of genomic DNA were used to characterize 45 methicillin-resistant coagulase-negative staphylococci (MRCoNS) isolates responsible of bacteremia recovered in patients at the Bone Marrow Transplant Centre of Tunisia in 1998-2007. Among the 45 MRCoNS isolates, Staphylococcus epidermidis was the most prevalent species (75. 6%) followed by Staphylococcus haemolyticus (22. 2%) and Staphylococcus hominis (2. 2%). Extended susceptibility profiles were generated for MRCoNS against 16 antimicrobial agents. Out of 45 mecA-positive strains, 43 (95. 6%) were phenotypically methicillin-resistant and two (4. 4%) were methicillin-susceptible. The msr(A) was the most prevalent gene (13 isolates; 48. 1%) among erythromycin-resistant isolates. The erm(C) was found alone in seven (25. 9%) or in combination with both erm(A) and erm(B) in two (7. 4%) isolates. The aac(6′)-Ie-aph(2″)-Ia was the most prevalent gene among aminoglycoside-resistant isolates, detected alone in 14 isolates (33. 3%) isolates, in combination with ant(4′)-Ia in 18 (42. 8%) isolates, in combination with aph(3′)-IIIa in four (9. 5%) or with both ant(4′)-Ia and aph(3′)-IIIa in two (4. 7%) isolates. The ant(4′)-Ia was detected in three (7. 1%) isolates and the aph(3′)-IIIa in one (2. 4%) isolate. Among tetracycline-resistant isolates, six (85. 7%) strains harbored the tet(K) gene and one (14. 3%) strain carried tet(K) and tet(M) genes. SCCmec types IV (31%) and III (24. 5%), the most prevalent types detected, were found to be more resistant to non-β-lactam antibiotics. A wide diversity of isolates was observed by PFGE among MRCoNS. © 2011 Institute of Microbiology, v.v.i, Academy of Sciences of the Czech Republic.
CITATION STYLE
Bouchami, O., Achour, W., Mekni, M. A., Rolo, J., & Hassen, A. B. (2011). Antibiotic resistance and molecular characterization of clinical isolates of methicillin-resistant coagulase-negative staphylococci isolated from bacteremic patients in oncohematology. Folia Microbiologica, 56(2), 122–130. https://doi.org/10.1007/s12223-011-0017-1
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