Isothermal Titration Calorimetry: A Powerful Tool for the Characterization of Molecular Interactions

Abstract

Isothermal titration calorimetry (ITC) is a well-established technique that allows the accurate and precise determination of binding equilibrium constants. It is able to provide detailed thermodynamic description of reacting systems without the need for van't Hoff analysis. ITC plays an important role in biology, biochemistry and medicinal chemistry, providing researchers with important information on the structure, stability and functionality of biological and synthetic molecules. This review demonstrates the power and versatility of ITC in providing accurate, rapid, and label-free measurement of the thermodynamics of molecular interactions. Moreover , this work focuses on recent studies employing ITC to investigate compounds of great biotechnological interest.