DETEKSI INFEKSI Human palillomavirus (HPV) 16/18 PADA KARSINOMA SEL SKUAMOSA RONGGA MULUT DENGAN NESTED PCR

Abstract

Head and neck cancer ranks fourth nationally cancer incidence in Indonesia. Oral SCC isone of Head and neck cancer incidence. Oral SCCrelated to several factors, includingsmoking, alcohol, viral infection human papillomavirus (HPV 16/18) and genetic On theother hand, HPV E6 oncoprotein binds and inactivates TP53, and result in loss of control ofthe cell cycle.This study aimed to detect HPV 16/18 infection in oral SCC. Detection of HPVserotypes 16 and 18 performed on FFPE DNA isolates oral SCC with the method of nestedPolymerase Chain Reaction (PCR). Nested PCR was performed in two stages, namelyamplification with L1 primer, followed by specific PCR E6 HPV-16 and HPV-18. A total of33% (11/33) FFPE samples showed positive for HPV 18 infection (single-sized DNA bands415bp) and not detected the presence of infection with HPV 16. It can be concluded that thetype of FFPE biosampel can be used for studies related to HPV infection. Furthermore, itshould be tested on different types biosampel by a larger amount so as to represent theprevalence of oncogenic HPV infection in Indonesia.