The Use of Meta-topolin in Cell and Tissue Cultures for Increasing Production of Secondary Metabolites

Abstract

Nowadays, increasing the amount of secondary metabolite, which is an active drug and food additive source, gradually gains importance in addition to its important role in the adaptation of plants to the environment. Plant cell and tissue culture technologies have been used to produce and increase plant secondary metabolites since the late 1960s. Strain improvement, medium optimization, selection of high production cell lines, and the use of elicitors such as plant growth regulators may lead to an increase in secondary metabolite production. Cytokinins are an important group of plant growth regulators that can affect plant growth in almost all stages and regulate various biotechnological processes. Meta-topolin (6-(3-hydroxybenzylamino)purine) is an aromatic natural cytokinin. It is thought that the production of secondary metabolites will increase and contribute to industrial production by adding meta-topolin growth regulator as an elicitor into the growth medium.