A novel electrochemical biosensor for the detection of uric acid and adenine

13Citations
Citations of this article
5Readers
Mendeley users who have this article in their library.

Abstract

A novel electrochemical biosensor for the detection of uric acid and adenine was prepared based on a gel containing multi-walled carbon nanotubes and room-temperature ionic liquid of 1-octyl-3-methylimidazolium hexafluorophosphate. The electrochemistry of uric acid and adenine was studied in this gel modified electrode. There was a significant two-way electrocatalytic activity upon both oxidation and reduction of uric acid. Similar to a bare glassy carbon electrode, uric acid undergoes a 2e,2H+ oxidation in phosphate buffer in the modified electrode. A diimine, the oxidation product of uric acid, was found to be an unstable intermediate, which was converted by a follow-up hydration reaction to an imine alcohol, with the reaction rate constant of 8.5 +/- 0.3 M-1·s-1 according to Nicholson's theory. Under optimum conditions, linear calibration graphs were obtained over the concentration range of 1.0×10-7 M - 1.0×10-5 M (uric acid) and 1.0×10-5 M - 6.0×10-4 M (adenine). Based on the signal-to-noise ratio of 3, the detection limits of the current technique was found to be as low as 9.0×10-8 M (uric acid) and 2.0×10-6 M (adenine), respectively. This novel biosensor was successfully applied for the assay of uric acid in human urine. Because of its good stability and long-term durability, such a gel modified electrode can provide a simple and easy approach for sensitive detection of uric acid and adenine.

Cite

CITATION STYLE

APA

Zhao, Y., Ye, T., Liu, H., Kou, Y., Li, M., Shao, Y., … Zhuang, Q. (2006). A novel electrochemical biosensor for the detection of uric acid and adenine. Frontiers in Bioscience, 11(SUPPL. 3), 2976–2982. https://doi.org/10.2741/2026

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free