Effect of formaldehyde and glutaraldehyde fixation on the immunochemical reactivity of potato virus A with monoclonal antibodies

Abstract

Potato virus A (PVA) was treated with different glutaraldehyde concentrations at a range of different pH values, and its immunochemical reactivity and integrity was tested in various types of ELISA using a panel of six monoclonal antibodies. Glutaraldehyde fixation was compared with formaldehyde fixation, and there was no significant difference in reactivity between PVA non-treated and treated with formaldehyde, but almost the entire immunochemical activity was lost after treating with glutaraldehyde.