BRCA1 regulates acetylation and ubiquitination of estrogen receptor-α

Abstract

Inherited mutations of the breast cancer susceptibility gene BRCA1 confer a high risk for breast cancer development. The 300RXKK and 266KXK motifs have been identified previously as sites for acetylation of the estrogen receptor-α (ER-α), and 302K was also found to be a site for BRCA1-mediated mono-ubiquitination of ER-α in vitro. Here we show that ER-α proteins with single or double lysine mutations of these motifs (including K303R, a cancer-associated mutant) are resistant to inhibition by BRCA1, even though the mutant ER-α proteins retain the ability to bind to BRCA1. We also found that BRCA1 overexpression reduced and knockdown increased the level of acetylated wild-type ER-α, without changing the total ER-α protein level. Increased acetylation of ER-α due to BRCA1 small interfering RNA was dependent upon phosphatidylinositol 3-kinase/Akt signaling and on up-regulation of the coactivator p300. In addition, using an in vitro acetylation assay, we found that in vitro-translated wild-type BRCA1 but not a cancer-associated point mutant (C61G) inhibited p300-mediated acetylation of ER-α. Furthermore, BRCA1 overexpression increased the levels of mono-ubiquitinated ER-α protein, and a BRCA1 mutant that is defective for ubiquitin ligase activity but retains other BRCA1 functions (I26A) did not ubiquitinate ER-α or repress its activity in vivo. Finally, ER-α proteins with mutations of the 300RXKK or 266KXK motifs showed modest or no BRCA1-induced ubiquitination. We propose a model in which BRCA1 represses ER-α activity, in part, by regulating the relative degree of acetylation vs. ubiquitination of ER-α. Copyright © 2010 by The Endocrine Society.