Optimization of mycelial growth of Xylaria papulis Lloyd (Xylariaceae) in indigenous liquid culture conditions, science city of Muñoz, Nueva Ecija, Philippines

Abstract

The search for potential and inexhaustible sources of bioactive compounds with great functional activities is imperative for potential drug leads in treating various human diseases. In this regard, this study investigated the optimum liquid medium and physical culture conditions for the mycelial growth of X. papulis Lloyd for nutraceutical studies. The greatest mycelial biomass was achieved in PSB liquid medium among the indigenous media evaluated, such as potato sucrose broth (PSB), corn meal broth (CMB), rice bran broth (RBB), and coconut water (CW). In terms of pH, X. papulis Lloyd was observed to grow in a wide range of pH (5.0−8.5), but the best mycelial growth was observed at pH 6.5. Room temperature of 28 °C, lighted (137 lux), and static conditions were the other optimum physical culture conditions for mycelial growth of X. papulis Lloyd.