Auto-calibration and micro-flow injection procedure based on automated hydrodynamic system for spectrophotometric determination of cobalt

Abstract

The development of analytical system has been focused on two aspects, first in the design of large versatile and robust equipment where operation is performed in a controlled laboratory. The second aspect developed in parallel, is focused on miniaturization and automation of analytical systems that can be used in the place where the sample is taken and generate results in real time. In this work, an automated hydrodynamic system was implemented with miniaturized actuators to provide a micro-flow injection and auto-calibration. The micro-flow injection procedure was operated exploited the multicommutation technique for handling solutions. The auto-calibration was operated by a virtual instrument allowing the response control process in real time. Automated micro-flow injection was developed to perform calibration. The autocalibration process, the precision and accuracy of the hydrodynamic system was evaluated by spectrophotometric determination of cobalt concentration. A linear response was observed from 0.1 to 3.0 mg L‑1. The result was validated with a commercial instrument.