The protocol described is intended to be used alongside molecular methods in order to reveal the relationship between the genome sequence and the biological properties of a single isolate of Citrus tristeza virus complex (CTV). It enables the phenotypic profile of the isolates to be defined and to infer the associated tristeza diseases (decline, seedling yellows, or stem pitting), to assess their aggressiveness or potential cross protectiveness (if any), and to monitor their movement into the host plants and the transmissibility by aphids.
CITATION STYLE
Russo, M., & Catara, A. F. (2019). Phenotyping Biological Properties of CTV Isolates. In Methods in Molecular Biology (Vol. 2015, pp. 15–27). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9558-5_3
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