Ecto‐Protein Kinase and Surface Protein Phosphorylation in PC12 Cells: Interactions with Nerve Growth Factor

Abstract

Abstract: The phosphorylation of surface proteins by ectoprotein kinase has been proposed to play a role in mechanisms underlying neuronal differentiation and their responsiveness to nerve growth factor (NGF). PC 12 clones represent an optimal model for investigating the mode of action of NGF in a homogeneous cell population. In the present study we obtained evidence that PC12 cells possess ectoprotein kinase and characterized the endogenous phosphorylation of its surface protein substrates. PC12 cells maintained in a chemically defined medium exhibited phosphorylation of proteins by [γ‐32P]ATP added to the medium at time points preceding the intracellular phosphorylation of proteins in cells labeled with 32Pi. This activity was abolished by adding apyrase or trypsin to the medium but was not sensitive to addition of an excess of unlabeled Pi. As also expected from ecto‐protein kinase activity, PC12 cells catalyzed the phosphorylation of an exogenous protein substrate added to the medium, dephospho‐α‐casein, and this activity competed with the endogenous phosphorylation for extracellular ATP. Based on these criteria, three protein components migrating in sodium dodecyl sulfate gels with apparent molecular weights of 105K, 39K, and 20K were identified as exclusive substrates of ecto‐protein kinase in PC12 cells. Of the phosphate incorporated into these proteins from extracellular ATP, 75–87% was found in phosphothreonine. The phosphorylation of the 39K protein by ecto‐protein kinase did not require Mg2+, implicating this activity in the previously demonstrated regulation of Ca2+‐dependent, high‐affinity norepinephrine uptake in PC12 cells by extracellular ATP. The protein kinase inhibitor K‐252a inhibited both intra‐ and extracellular protein phosphorylation in intact PC12 cells. Its hydrophilic analogue K‐252b, had only minimal effects on intracellular protein phosphorylation but readily inhibited the phosphorylation of specific substrates of ecto‐protein kinase in PC12 cells incubated with extracellular ATP, suggesting the involvement of ecto‐protein kinase in the reported inhibition of NGF‐induced neurite extension by K‐252b. Preincubation of PC12 cells with 50 ng/ml of NGF for 5 min stimulated the activity of ecto‐protein kinase toward all its endogenous substrates. Exposure of PC12 cells to the same NGF concentration for 3 days revealed another substrate of ecto‐protein kinase, a 53K protein, whose surface phosphorylation is expressed only after NGF‐induced neuronal differentiation. In the concentration range (10–100 μM) at which 6‐thioguanine blocked NGF‐promoted neurite outgrowth in PC12 cells, 6‐thioguanine effectively inhibited the phosphorylation of specific proteins by ecto‐protein kinase. This study provides the basis for continued investigation of the involvement of ecto‐protein kinase and its surface protein substrates in neuronal differentiation, neuritogenesis, and synaptogenesis. Copyright © 1993, Wiley Blackwell. All rights reserved