Noncoding regulatory genomic elements are central for cellular function, differentiation, and disease, but remain poorly characterized. FAIRE (formaldehyde-assisted isolation of regulatory elements) has emerged as a simple method to identify and analyze active regulatory sequences based on their decreased nucleosomal content. More recently FAIRE was combined with high-throughput sequencing (FAIRE-seq) to locate tissue-specific regulatory elements at a genome scale in purified human pancreatic islets. Here we describe the implementation of the FAIRE method in human pancreatic islet cells.
CITATION STYLE
Nammo, T., Rodríguez-Seguí, S. A., & Ferrer, J. (2011). Mapping Open Chromatin with Formaldehyde-Assisted Isolation of Regulatory Elements. In Methods in Molecular Biology (Vol. 791, pp. 287–296). Humana Press Inc. https://doi.org/10.1007/978-1-61779-316-5_21
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