RNA interference (RNAi) is a biological process by which double-stranded RNA (dsRNA) induces sequence-specific gene silencing by targeting mRNA for degradation. As a tool for knocking down the expression of individual genes posttranscriptionally, RNAi has been widely used to study the cellular function of genes. In this chapter, I describe procedures for using gene-specific, synthetic, short interfering RNA (siRNA) to induce gene silencing in mammalian cells. Protocols for using lipid-based transfection reagents and electroporation techniques are provided. Potential challenges and problems associated with the siRNA technology are also discussed.
CITATION STYLE
Han, H. (2018). RNA interference to knock down gene expression. In Methods in Molecular Biology (Vol. 1706, pp. 293–302). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7471-9_16
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