Effects of low ozone exposure of spring wheat on net CO2 uptake, Rubisco, leaf senescence and grain filling

Abstract

Effects of ozone on spring wheat (Triticum aestivum L. cv. Satu) were studied in an open-top chamber experiment during two growing seasons (1992-1993) at Jokioinen in south-west Finland. The wheat was exposed to filtered air (CF), non-filtered air (NF), non-filtered air + 35 nl l-1 ozone for 8 h d-1 (NF+) and ambient air (AA). Each treatment was replicated five times. Two wk after anthesis, after 4 wk of ozone treatment (NF+, 45 nl l-1 1000-1800 hours, seasonal mean) the net CO2 uptake of wheat flag leaves was decreased by c. 40% relative to CF and NF treatments, both initial and total activity of Rubisco and the quantity of protein-bound SH groups were decreased significantly. Added ozone also significantly accelerated flag leaf senescence recorded as a decrease in chloroplast size. The effect was significant 2 wk after anthesis, and senescence was complete after 4 wk. In the CF and NF treatments senescence was complete 5 wk after anthesis. The significant effect of ozone on the chloroplasts and net CO2 uptake 2 wk after anthesis did not affect the grain filling rate. However, since the grain filling period was shorter for ozone fumigated plants, kernels were smaller. The decrease in 1000-grain weight explained most of the yield reduction in the plants under NF+ treatment. The results indicate that wheat plants are well buffered against substantial decrease in source activity, and that shortened flag leaf duration is the major factor causing ozone-induced yield loss.