Pilin processing follows a different temporal route than that of archaellins in methanococcus maripaludis

7Citations
Citations of this article
9Readers
Mendeley users who have this article in their library.

Abstract

Methanococcus maripaludis has two different surface appendages: type IV-like pili and archaella. Both structures are believed to be assembled using a bacterial type IV pilus mechanism. Each structure is composed of multiple subunits, either pilins or archaellins. Both pilins and archaellins are made initially as preproteins with type IV pilin-like signal peptides, which must be removed by a prepilin peptidase-like enzyme. This enzyme is FlaK for archaellins and EppA for pilins. In addition, both pilins and archaellins are modified with N-linked glycans. The archaellins possess an N-linked tetrasaccharide while the pilins have a pentasaccharide which consists of the archaellin tetrasaccharide but with an additional sugar, an unidentified hexose, attached to the linking sugar. In this report, we show that archaellins can be processed by FlaK in the absence of N-glycosylation and N-glycosylation can occur on archaellins that still retain their signal peptides. In contrast, pilins are not glycosylated unless they have been acted on by EppA to have the signal peptide removed. However, EppA can still remove signal peptides from non-glycosylated pilins. These findings indicate that there is a difference in the order of the posttranslational modifications of pilins and archaellins even though both are type IV pilin-like proteins.

References Powered by Scopus

Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications

48001Citations
N/AReaders
Get full text

Methanogens: reevaluation of a unique biological group

2244Citations
N/AReaders
Get full text

Structure and biosynthesis of prokaryotic glycoproteins

203Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Archaeal type IV pili and their involvement in biofilm formation

66Citations
N/AReaders
Get full text

Archaeal cell surface biogenesis

53Citations
N/AReaders
Get full text

Identification of the first transcriptional activator of an archaellum operon in a euryarchaeon

24Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Nair, D. B., & Jarrell, K. F. (2015). Pilin processing follows a different temporal route than that of archaellins in methanococcus maripaludis. Life, 5(1), 85–101. https://doi.org/10.3390/life5010085

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 4

80%

Researcher 1

20%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 2

40%

Biochemistry, Genetics and Molecular Bi... 1

20%

Computer Science 1

20%

Chemistry 1

20%

Save time finding and organizing research with Mendeley

Sign up for free