The aim of this study is the analysis and characterization of a hydrolyzed keratin-based biomaterial and its processing using electrospinning technology to develop in vitro tissue models. This biomaterial, extracted from poultry feathers, was mixed with type A porcine gelatin and cross-linked with γ-glycidyloxy-propyl-trimethoxy-silane (GPTMS) to be casted initially in the form of film and characterized in terms of swelling, contact angle, mechanical properties, and surface charge density. After these chemical-physical characterizations, electrospun nanofibers structures were manufactured and their mechanical properties were evaluated. Finally, cell response was analyzed by testing the efficacy of keratin-based structures in sustaining cell vitality and proliferation over 4 days of human epithelial, rat neuronal and human primary skin fibroblast cells.
CITATION STYLE
Fortunato, G. M., Da Ros, F., Bisconti, S., De Acutis, A., Biagini, F., Lapomarda, A., … Vozzi, G. (2019). Electrospun structures made of a hydrolyzed keratin-based biomaterial for development of in vitro tissue models. Frontiers in Bioengineering and Biotechnology, 7(JUL). https://doi.org/10.3389/fbioe.2019.00174
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