Characterization of the bovine κ-casein gene promoter

Abstract

κ-Casein gene promoter was localized within a 570-bp fragment (−552/+ 18) of a 5′-flanking region by the gene transfection assay. Deletion mutation analysis in mammary epithelial cell line, HC11, suggested that there are regulatory element in a region from −439 through −125. Some nuclear proteins from lactating rate mammary gland bind to this region specifically. One of them expressed preferentially during pregnancy bound to a 132-bp fragment (−439/ −308) and another expressed preferentially during lactation bound to a 183-bp fragment (−307/ −125). © 1996, Taylor & Francis Group, LLC. All rights reserved.