Antioxidant and anticholinesterase activities of Juglans regia L. Growing in Turkey

Abstract

Objective: World's ageing population continues to grow older together with high percentage prevalence of Alzheimer's disease encourage science to find herbs or biologic compounds which can be utilized for it prophylaxis. Juglans regia L. is not simply an agricultural product, its fruits, leaves, barks, stems, pericarps, and flowers are all utilized for diversified medicinal usages. In the present investigation, we examined the anticholinesterase and antioxidant capacities of the lyophilized aqueous extracts of leaves from J. regia. Material and Method: Phenolic contents and antioxidant activity were assessed by Folin-Ciocalteu's, qualitative/quantitative DPPH and TBA methods. Anticholinesterase activity was assessed by Ellman’s method. Result and Discussion: The total phenolic content was found 478.32 mg/g. The extract indicated antioxidant activity with DPPH test (IC50: 123.66 μg/mL) and TBA assay (IC50: 209.23 μg/mL). Moreover, the extract of J. regia indicated remarkable inhibition towards acetylcholinesterase (40.09%) and butyrylcholinesyterase (56.23%) enzymes. These data propose that J. regia can be utilised a potency for pharmaceutical products which get antioxidant and anticholinesterase activity.