Identification and Characterization of a Novel Retinoic Acid Response Element in Zebrafish cyp26a1 Promoter

17Citations
Citations of this article
23Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Cyp26A1 is a major enzyme that controls retinoic acid (RA) homeostasis by metabolizing RA into bio-inactive metabolites. Previously, we demonstrated that zebrafish cyp26a1 promoter possesses two conserved RA response elements (RAREs; proximal R1 and distal R2) in response to RA. Here, we report that it contains a novel RARE (R3) lying between R1 and R2. Mutagenesis analysis reveals that R3 works together with R1 and R2 to ensure the maximum RA inducibility of cyp26a1 promoter. Performing electrophoretic mobility shift assay and chromatin immunoprecipitation assay, we show that RA receptor alpha can bind the novel RARE. Creating and analyzing transgenic zebrafish of Tg(cyp26a1-R3mut:eYFP)nju3/+ that harbor enhanced yellow fluorescent protein reporter gene (eYFP) driven by cyp26a1 promoter with mutated R3, we demonstrate that the reporter is mainly expressed in tissues of endogenous RA independent but not regions of RA dependent. Like Tg(cyp26a1:eYFP)nju1/+, which harbor eYFP driven by wild-type cyp26a1 promoter, the reporter in Tg(cyp26a1-R3mut:eYFP)nju3/+ responds to excessive RA dose dependently. However, it is expressed in a significantly lower level than the reporter in Tg(cyp26a1:eYFP)nju1/+ in response to exogenous RA. Taken together, our results demonstrate that zebrafish cyp26a1 promoter contains a novel RARE that plays crucial roles in regulating cyp26a1 expression during early development of zebrafish. © 2011 Wiley Periodicals, Inc.

References Powered by Scopus

A transposon-mediated gene trap approach identifies developmentally regulated genes in zebrafish

708Citations
N/AReaders
Get full text

Retinoic acid in development: Towards an integrated view

583Citations
N/AReaders
Get full text

The retinoic acid-metabolizing enzyme, CYP26A1, is essential for normal hindbrain patterning, vertebral identity, and development of posterior structures

475Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Zebrafish: A Resourceful Vertebrate Model to Investigate Skeletal Disorders

89Citations
N/AReaders
Get full text

Input overload: Contributions of retinoic acid signaling feedback mechanisms to heart development and teratogenesis

38Citations
N/AReaders
Get full text

Excessive feedback of Cyp26a1 promotes cell non-autonomous loss of retinoic acid signaling

35Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Li, J., Hu, P., Li, K., & Zhao, Q. (2012). Identification and Characterization of a Novel Retinoic Acid Response Element in Zebrafish cyp26a1 Promoter. Anatomical Record, 295(2), 268–277. https://doi.org/10.1002/ar.21520

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 11

85%

Professor / Associate Prof. 1

8%

Researcher 1

8%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 8

57%

Biochemistry, Genetics and Molecular Bi... 3

21%

Medicine and Dentistry 2

14%

Chemical Engineering 1

7%

Save time finding and organizing research with Mendeley

Sign up for free