Abstract
Methods that quickly assess microbial density and aggregation in soil and sediments are needed in environmental microbiology. We report a flow cytometry method that uses the green and orange emission of the fluorochrome SYTO-13 to discriminate between bacteria and clay-humic acid particles. This approach distinguishes single or clustered bacteria, and clusters of bacteria and abiotic particles during the growth of the biosurfactant-producing strain Pseudomonas aeruginosa 19SJ on solid phenanthrene in the presence of humic acid-clay complexes. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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CITATION STYLE
Resina-Pelfort, O., GarcÃa-Junco, M., Ortega-Calvo, J. J., Comas-Riu, J., & Vives-Rego, J. (2006). Flow cytometry discrimination between bacteria and clayâhumic acid particles during growth-linked biodegradation of phenanthrene by Pseudomonas aeruginosa 19SJ. FEMS Microbiology Ecology, 43(1), 55–61. https://doi.org/10.1111/j.1574-6941.2003.tb01045.x
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