Analysis on Alu-I growth hormone (GHAlu-I) gene in Bali cattle

Abstract

The research was conducted to identify Alu-I locus of growth hormone (GH) gene in Bali cattle by using 232 blood samples collected from Bali and Lombok islands. PCR-RFLP and sequencing methods were used to detect the polymorphism and nucleotide sequence at Alu-I locus of GH gene. The result showed that Bali cattle from Bali island has one genotype (LL genotype), whereas Bali cattle originating from Lombok island has two genotypes, namely LL and VV genotypes, respectively. The L and V allele frequencies from Bali and Lombok islands were 1.00 and 0.00; 0.99 and 0.01, respectively. The sequencing result of Bali cattle LL genotype showed an AGCT sequence of enzyme Alu-I restriction site. Based on polymorphic informative content (PIC) value, it can be concluded that Alu-I locus of Bali cattle from Bali and Lombok islands were monomorphic and polymorphic, respectively.