SYNTHESIS AND CHARACTERIZATION OF A NOVEL BONE GRAFT MATERIAL CONTAINING BIPHASIC CALCIUM PHOSPHATE AND CHITOSAN FORTIFIED WITH ALOE VERA

Abstract

The bone grafts were prepared using nanohydroxyapatite (nanoHAP) with the extracts of AV and mixed with beta tricalcium phosphate (βTCP) in a ratio of 60:40. The formed BCP was then conjugated with CH to form the bone graft. The Phytochemicals of AV imparts an anti-inflammatory and wound healing property to the bonegraft in addition to its osteoconductivity. The bone graft was subjected to various conventional characterization techniques like FTIR, XRD, SEM, UV spectrophotometric analysis, compression and tensile strength to reveal its chemical composition, surface morphology and mechanical strength. The UV spectrophotometric analysis and SEM images revealed the mineral deposition on the bone graft after immersing in SBF, thereby increasing its ossification property. FTIR and XRD revealed the presence of BCP with nano HAP crystals and Chitosan in the graft. Compression and tensile strength of the graft showed that the graft can be used in non–load bearing areas. In this study, a bone graft was synthesized using a novel technique of introducing the extracts of AV in the graft along with BCP and CH. The results revealed that the bone graft can be used as an Osteoconductive material for various biomedical applications.