In vitro stimulation of CD34+ cells with IL-2 induces NK cell differentiation. In order to define the stages of NK cell development, which influence their generation from CD34 cells, we cultured GCSF mobilized peripheral blood CD34+ cells in the presence of stem cell factor and IL-2. After three weeks culture we found a diversity of CD56+ subsets which possessed granzyme A, but lacked the cytotoxic apparatus required for classical NK-like cytotoxicity. However, these CD56+ cells had the unusual property of inhibiting proliferation of K562 and P815 cell lines in a cell-contact dependent fashion. © 2005 Sconocchia et al; licensee BioMed Central Ltd.
CITATION STYLE
Sconocchia, G., Provenzano, M., Rezvani, K., Li, J., Melenhorst, J., Hensel, N., & Barrett, A. J. (2005). CD34+ cells cultured in stem cell factor and interleukin-2 generate CD56+ cells with antiproliferative effects on tumor cell lines. Journal of Translational Medicine, 3. https://doi.org/10.1186/1479-5876-3-15
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