Protoplasts: a friendly tool to study aluminum toxicity and coffee cell viability

Abstract

Objective: Aluminum toxicity is a major limiting factor with regard to crop production and quality in most acidic soils around the world. We propose the use of C. arabica L. protoplasts to evaluate the toxic effects of aluminum, the nuclear localization of aluminum and propensity of aluminum to cause DNA damage. Results: After protoplasts were exposed to aluminum (Al) for varying periods of time (0, 5, 10, 20 and 30 min), we detected a reduction in protoplast viability. Additionally, we observed a rapid decline in the ability of protoplasts to synthesize DNA following exposure to Al for 30 min. Furthermore, DNA damage was observed after 10 min of treatment with Al. Conclusions: Protoplasts can be used to evaluate the effects of Al upon entry into the cell, which affects the structure of the nucleus. These results indicate that protoplasts provide a useful model for the study Al toxicity at the cellular level.