Experimental study on the effect of vitamin C administration on lipid peroxidation and antioxidant enzyme activity in rats exposed to chlorpyriphos and lead acetate

Abstract

Aim: To evaluate the effects of chlorpyriphos, lead acetate, vitamin C alone, and in combination on the activity of oxidative stress parameters in wistar rats. Marerial and Methods: Rats of 150-200g body weight were divided into eight groups of six animals each and were subjected to various daily oral treatment regimes for 98 days. Group I served as control receiving only corn oil, group II received chlorpyriphos @ 5.5 mg/kg in corn oil, group III received lead acetate @100 ppm in water, whereas animals in group IVth received a combination of chlorpyriphos @ 5.5mg/kg in corn oil and lead acetate @ 100 ppm in water. Group Vth received vitamin C @ 100mg/kg in water, group VIth received a combination of chlorpyriphos @ 5.5mg/kg and vitamin C @ 100mg/kg, group VIIth received lead acetate @ 100 ppm in water and vitamin C @ 100mg/kg and group VIIIth received chlorpyriphos @ 5.5mg/kg, lead acetate @100ppm in water and vitamin C @ 100mg/kg. Results: Administration of both chlorpyriphos and lead acetate caused a significant decrease in oxidative stress parameters viz. blood glutathione, catalase, superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione-s-transferase (GST) along with a significant increase in lipid peroxidation level when given alone or in combination. Conclusions: The study demonstrated that treatment of chlorpyriphos and lead treated rats with vitamin C significantly improved some of altered oxidative stress parameters revealing the protective effect of this vitamin C against oxidative stress induced by chlorpyriphos and lead.