Temperature-Dose Relationships with Aflatoxin on the Brine Shrimp, Artemia salina

Abstract

Temperature-dose relationships with aflatoxin B1 were studied, using the brine shrimp, Artemia salina. Increase in temperature resulted in increased sensitivity by the brine shrimp to aflatoxin. Optimum sensitivity occurred at 37.5°C. Positive results were obtained at 0.5 μg aflatoxin B1/ml artificial sea water with a mortality of over 60%. Greater than 90% mortality occurred at dose levels of 1.0 μ/ml and above. The test can be conducted in 24 hours, and highly trained personnel are unnecessary.