An isolate of Apple stem grooving virus associated with Cleopatra mandarin fruit intumescence

Abstract

A citrus tatter leaf isolate (CTLV-Cl) of Apple stem grooving virus (ASGV) has been found to be associated with a fruit rind intumescence in Cleopatra mandarin (Citrus reshni) in Limeira (SP). The CTLV-Cl was mechanically transmitted to the main experimental herbaceous hosts of CTLV. Chenopodium quinoa and C. amaranticolor reacted with local lesions and systemic symptoms while other test plants reacted somewhat differently than what is reported for CTLV. A pair of primers designed for specific detection of ASGV and CTLV amplified the expected 801 bp fragment from the CTLV-Cl-infected plants. Typical capillovirus-like particles were observed by the electron microscope in experimentally infected C. quinoa and C. amaranticolor leaves.A presença de intumescências em frutos de tangerina Cleópatra (Citrus reshni) observadas em Limeira (SP), foram associadas ao Citrus tatter leaf (CTLV-Cl), um isolado do virus do acanalamento do lenho da maceira (Apple stem grooving virus, ASGV). O CTLV-Cl foi transmitido mecanicamente para as principais hospedera experimentais do CTLV. Chenopodium quinoa e C. amaranticolor reagiram com lesões locais e sintomas sistêmicos, enquanto as demais reagiram ligeiramente diferente do relatado para CTLV. Um par de primers desenhados para CTLV e ASGV amplificaram um fragmento de 801 pb, conforme o esperado, a parteir de plantas herbaceas infetadas com o CTLV-Cl. Particulas do tipo capillovirus foram observadas ao microscópio eletrônico, em folhas de Chenopodium quinoa e C. amaranticolor experimentalmente infetadas.