Crystal structure of the Rad3/XPD regulatory domain of Ssl1/p44

Abstract

Background: Ssl1/p44 is a critical helicase stimulating factor for Rad3/XPD. Results: Structure-based mutation analysis of the β4-α5 loop of Ssl1/p44 resulted in defects of Rad3/XPD stimulation and yeast cell lethality. Conclusion: The β4-α5 loop of Ssl1/p44 is essential for the functional regulation of Rad3/XPD. Significance: These findings provide insights into the interaction between Rad3/XPD and Ssl1/p44 in TFIIH complex.