Total phenolic, total flavonoid and phytochemical screening by FTIR spectroscopic of standardized extract of Mikania micrantha leaf

Abstract

Background: Mikania micrantha is a great plant that has been used as raw material for traditional medicines. Objective: This paper aims to evaluate total phenols, total flavonoids, and phytochemical screening by FTIR spectroscopy of standardized extract of Mikania micrantha leaf to confirm its medicinal values. Materials and Methods: The leaves were extracted by maceration method using ethanol 96% and evaporated by rotary evaporator. The determination of total phenolic and total flavonoid were performed by spectroscopic method. The phytochemical constituent was screened through the bioactive group of the chemical by FTIR analysis. Results: The total phenolic of extract of Mikania micrantha leaf ranged from 13.19±0.74 to 34.24±1.24 mg gallic acid equivalent/g and total flavonoid ranged from 1.11±0.11 to 20.63±0.16 mg quercetin/g. FTIR analysis confirmed the presence of O-H, aliphatic CH, and C=O functional group. Conclusion: the result of this study confirm that Mikania micrantha possesses the potential of bioactive compounds which are responsible for the biological activities that are useful for raw material of traditional medicines.