Cytogenetic analysis of translocations in soybean

Abstract

A complete tester set of translocations would be useful in the advancement of classical and molecular genetic linkage maps in soybean. Seven translocation lines have been identified in soybean. Establishment of the independence of these seven translocation lines is a necessary step toward isolation of other independent translocations. This study confirmed cytologically that the seven lines are true chromosome translocations. Fourteen F1 progeny from intercrosses of six of the seven translocation lines were analyzed cytologically. Results suggested that 6 of the 20 chromosomes are involved in reciprocal translocations in the six lines analyzed. The translocations Clark T/T, KS172-11-3, and KS175-7-3 share one chromosome in common, while PI 189866, KS171-31-2, and L75-0283-4 share a different chromosome in common. Percentage pollen abortion, ovule abortion, and reduction in seed set were higher in F1 progeny from crosses among homozygous translocation lines (a greater number of interchanged chromosomes) than from crosses between homozygous translocation lines and normal chromosome lines (only two nonhomologous chromosomes translocated). In terms of pollen and ovule abortion, and cytological behavior, soybean translocations were similar to those reported for many other crop species.