Histological Estimation of Age at Death from the Compact Bone of Burned and Unburned Human Ribs

Abstract

This study describes the estimation of age at death from the compact bone of burned and unburned human ribs. Bone samples came from individuals of known age, sex, and cause of death. Each bone was divided into four sections; three sections were burned at 700, 800, and 1000°C. Undecalcified, unstained ground cross sections were photographed, and 28 variables were analyzed in the bones using SigmaScan Pro 5. Age-related as well as heat-induced microstructural changes were found. These changes were often very similar and made estimating the age at death difficult in the burned bones. Differences between the sexes were found in some variables, caused by both aging and also by the different behavior of some variables during burning. Regression equations were developed to estimate age at death for unburned bones (r2 = 0.579 and 0.707), bones burned at 700°C (r2 = 0.453 and 0.501), and 800°C (r2 = 0.334 and 0.340). © 2012 American Academy of Forensic Sciences.