A TREK-1-like potassium channel in atrial cells inhibited by β-adrenergic stimulation and activated by volatile anesthetics

Abstract

Many members of the two-pore-domain potassium (K+) channel family have been detected in the mammalian heart but the endogenous correlates of these channels still have to be identified. We investigated whether IKAA, a background K+ current activated by negative pressure (stretch) and by arachidonic acid (AA) and sensitive to intracellular acidification, could be the native correlate of TREK-1 in adult rat atrial cells. Using the inside-out configuration of the patch-clamp technique, we found that IKAA, like TREK-1, was outwardly rectifying in physiological K+ conditions, with a conductance of 41 pS at +50 mV. Like TREK-1, IKAA was reversibly activated by clinical concentrations of volatile anesthetics (in mmol/L, chloroform 0.18, halothane 0.11, and isoflurane 0.69). In cell-attached experiments, IKAA was inhibited by chlorophenylthio-cAMP (500 μmol/L]) and also by stimulation of ǧ-adrenergic receptors with isoproterenol (1 μmol/L). In addition, TREK-1 mRNAs were detected in all cardiac tissues, and the TREK-1 protein was immunolocalized in isolated atrial myocytes. Such a background potassium channel might contribute to the positive inotropic effects produced by β-adrenergic stimulation of the heart. It might also be involved in the regulation of the atrial natriuretic peptide secretion.