Flow cytometry is an essential tool for studying the tumor-immune microenvironment. It allows us to quickly quantify and identify multiple cell types in a heterogeneous sample. This chapter provides an overview of the flow cytometry instrumentation and a discussion of the appropriate considerations and steps in building a reproducible flow cytometry staining panel. We present an updated lymphoid tissue and solid tumor-infiltrating leucocyte flow cytometry staining protocol and an example of flow cytometry data analysis.
CITATION STYLE
Santinon, F., Young, Y. K., del Rincón, S. V., & Mann, K. K. (2023). Analyzing the Tumor-Immune Microenvironment by Flow Cytometry. In Methods in Molecular Biology (Vol. 2614, pp. 17–36). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2914-7_2
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