The visualization of meiotic chromosomes and their associated protein structures in both wild-type and mutant cells adds valuable insight into the molecular pathways that underlie reproductive cell formation. Here we describe basic methodology for visualizing meiotic chromosomes in a long-standing model organism for investigating the molecular and cell biology of meiosis, the budding yeast, S. cerevisiae. This chapter furthermore highlights a variety of conditional expression regimes that can be used to understand the dynamics and/or developmental constraints of chromosomal protein structures; such dynamic aspects of the macromolecular structures that mediate meiotic chromosome biology are typically not obvious from standard protein visualization experiments.
CITATION STYLE
MacQueen, A. J., & Rockmill, B. (2017). Analysis of meiotic chromosome-associated protein dynamics using conditional expression in budding yeast. In Methods in Molecular Biology (Vol. 1471, pp. 157–174). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6340-9_8
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